e coli k 12 Search Results


e coli mg1655 rare  (Addgene inc)
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rat trka intracellular domain  (Addgene inc)
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chromosomal terminus  (Addgene inc)
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Chromosomal Terminus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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midreplichore  (Addgene inc)
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Midreplichore, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli k-12 mg1655  (Marinus)
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E. Coli K 12 Mg1655, supplied by Marinus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli k12 arrays  (Ocimum Biosolutions)
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Ocimum Biosolutions e. coli k12 arrays
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e. coli k-12 v2 ocichip microarray slides  (Ocimum Biosolutions)
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E. Coli K 12 V2 Ocichip Microarray Slides, supplied by Ocimum Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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keio collection of 3884 e. coli k-12 in-frame single-gene knockout mutants  (BioResource International Inc)
90
BioResource International Inc keio collection of 3884 e. coli k-12 in-frame single-gene knockout mutants
(A) Mutants with increased resistance to PGRP killing were identified by three-stage screening of the entire <t>Keio</t> collection of single gene deletion mutants (Fig. S1 and Table S1) and here the survival of the parental strain (BW25113) and mutants following 3-hr incubation with 200 µg/ml of BSA (as a control) or PGRP is shown. Gene products, their functions, and numerical data are shown in Table S1. (B) Mutants for the key genes for the respiratory chain and TCA cycle, and their regulators (cyaA and crp) were constructed in MG1655 and their sensitivity to killing by 100 µg/ml of PGRP was similarly tested. The results are means of 3 experiments, expressed as percent of initial inoculum (100%) + SEM; ^ P≤0.05, ^^ P<0.001, numbers of <t>viable</t> <t>bacteria</t> (colony forming units) of Δ mutants versus parental strain (t-test).
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strains mg1655 (k-12 f- λ- rph-1)  (BioResource International Inc)
90
BioResource International Inc strains mg1655 (k-12 f- λ- rph-1)
(A) Mutants with increased resistance to PGRP killing were identified by three-stage screening of the entire <t>Keio</t> collection of single gene deletion mutants (Fig. S1 and Table S1) and here the survival of the parental strain (BW25113) and mutants following 3-hr incubation with 200 µg/ml of BSA (as a control) or PGRP is shown. Gene products, their functions, and numerical data are shown in Table S1. (B) Mutants for the key genes for the respiratory chain and TCA cycle, and their regulators (cyaA and crp) were constructed in MG1655 and their sensitivity to killing by 100 µg/ml of PGRP was similarly tested. The results are means of 3 experiments, expressed as percent of initial inoculum (100%) + SEM; ^ P≤0.05, ^^ P<0.001, numbers of <t>viable</t> <t>bacteria</t> (colony forming units) of Δ mutants versus parental strain (t-test).
Strains Mg1655 (K 12 F λ Rph 1), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli (k-12) cells  (Fisher Scientific)
90
Fisher Scientific e. coli (k-12) cells
(A) Mutants with increased resistance to PGRP killing were identified by three-stage screening of the entire <t>Keio</t> collection of single gene deletion mutants (Fig. S1 and Table S1) and here the survival of the parental strain (BW25113) and mutants following 3-hr incubation with 200 µg/ml of BSA (as a control) or PGRP is shown. Gene products, their functions, and numerical data are shown in Table S1. (B) Mutants for the key genes for the respiratory chain and TCA cycle, and their regulators (cyaA and crp) were constructed in MG1655 and their sensitivity to killing by 100 µg/ml of PGRP was similarly tested. The results are means of 3 experiments, expressed as percent of initial inoculum (100%) + SEM; ^ P≤0.05, ^^ P<0.001, numbers of <t>viable</t> <t>bacteria</t> (colony forming units) of Δ mutants versus parental strain (t-test).
E. Coli (K 12) Cells, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli k-12 strain w3110  (BioResource International Inc)
90
BioResource International Inc e. coli k-12 strain w3110
(A) Mutants with increased resistance to PGRP killing were identified by three-stage screening of the entire <t>Keio</t> collection of single gene deletion mutants (Fig. S1 and Table S1) and here the survival of the parental strain (BW25113) and mutants following 3-hr incubation with 200 µg/ml of BSA (as a control) or PGRP is shown. Gene products, their functions, and numerical data are shown in Table S1. (B) Mutants for the key genes for the respiratory chain and TCA cycle, and their regulators (cyaA and crp) were constructed in MG1655 and their sensitivity to killing by 100 µg/ml of PGRP was similarly tested. The results are means of 3 experiments, expressed as percent of initial inoculum (100%) + SEM; ^ P≤0.05, ^^ P<0.001, numbers of <t>viable</t> <t>bacteria</t> (colony forming units) of Δ mutants versus parental strain (t-test).
E. Coli K 12 Strain W3110, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Mutants with increased resistance to PGRP killing were identified by three-stage screening of the entire Keio collection of single gene deletion mutants (Fig. S1 and Table S1) and here the survival of the parental strain (BW25113) and mutants following 3-hr incubation with 200 µg/ml of BSA (as a control) or PGRP is shown. Gene products, their functions, and numerical data are shown in Table S1. (B) Mutants for the key genes for the respiratory chain and TCA cycle, and their regulators (cyaA and crp) were constructed in MG1655 and their sensitivity to killing by 100 µg/ml of PGRP was similarly tested. The results are means of 3 experiments, expressed as percent of initial inoculum (100%) + SEM; ^ P≤0.05, ^^ P<0.001, numbers of viable bacteria (colony forming units) of Δ mutants versus parental strain (t-test).

Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: (A) Mutants with increased resistance to PGRP killing were identified by three-stage screening of the entire Keio collection of single gene deletion mutants (Fig. S1 and Table S1) and here the survival of the parental strain (BW25113) and mutants following 3-hr incubation with 200 µg/ml of BSA (as a control) or PGRP is shown. Gene products, their functions, and numerical data are shown in Table S1. (B) Mutants for the key genes for the respiratory chain and TCA cycle, and their regulators (cyaA and crp) were constructed in MG1655 and their sensitivity to killing by 100 µg/ml of PGRP was similarly tested. The results are means of 3 experiments, expressed as percent of initial inoculum (100%) + SEM; ^ P≤0.05, ^^ P<0.001, numbers of viable bacteria (colony forming units) of Δ mutants versus parental strain (t-test).

Article Snippet: Bacteria, growth, and media The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Incubation, Control, Construct, Bacteria

(A) Time kinetics of changes of H2O2 in E. coli MG1655 treated with 100 µg/ml BSA or PGRP, or with 100 µM paraquat. (B) H2O2 in E. coli BW25113 and in the indicated deletion mutants from Keio collection treated with 100 µg/ml BSA or PGRP for 15 min. (C) H2O2 in E. coli MG1655 and in the indicated deletion mutants constructed in our laboratory treated with 100 µg/ml BSA or PGRP for 15 min. The results are means of 3–4 experiments ± SEM (SEM were within symbols if not visible); * P<0.05, ** P<0.001, PGRP vs BSA; +P<0.05, ++P<0.001, paraquat vs BSA; ^ P<0.05, ^^ P<0.001, PGRP-treated mutant vs parental strain.

Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: (A) Time kinetics of changes of H2O2 in E. coli MG1655 treated with 100 µg/ml BSA or PGRP, or with 100 µM paraquat. (B) H2O2 in E. coli BW25113 and in the indicated deletion mutants from Keio collection treated with 100 µg/ml BSA or PGRP for 15 min. (C) H2O2 in E. coli MG1655 and in the indicated deletion mutants constructed in our laboratory treated with 100 µg/ml BSA or PGRP for 15 min. The results are means of 3–4 experiments ± SEM (SEM were within symbols if not visible); * P<0.05, ** P<0.001, PGRP vs BSA; +P<0.05, ++P<0.001, paraquat vs BSA; ^ P<0.05, ^^ P<0.001, PGRP-treated mutant vs parental strain.

Article Snippet: Bacteria, growth, and media The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Construct, Mutagenesis

Parental E. coli or the indicated deletion mutants from Keio collection (BW25113) or from our laboratory (MG1655) were treated with 100 µg/ml BSA or PGRP for 15 min (A) or 5 min (B and C). The results are means of 3–4 experiments + SEM; * P<0.05, ** P<0.001, PGRP vs BSA; ^ P<0.05, ^^ P<0.001, mutant vs parental strain.

Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: Parental E. coli or the indicated deletion mutants from Keio collection (BW25113) or from our laboratory (MG1655) were treated with 100 µg/ml BSA or PGRP for 15 min (A) or 5 min (B and C). The results are means of 3–4 experiments + SEM; * P<0.05, ** P<0.001, PGRP vs BSA; ^ P<0.05, ^^ P<0.001, mutant vs parental strain.

Article Snippet: Bacteria, growth, and media The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Mutagenesis

E. coli and the indicated deletion mutants from Keio collection (BW25113) or from our laboratory (MG1655) were treated with 100 µg/ml BSA or PGRP for 30 min. The results are means of 3 experiments + SEM; * P<0.05, ** P<0.001, PGRP vs BSA; ^ P<0.05, mutant vs parental strain.

Journal: Molecular microbiology

Article Title: Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism

doi: 10.1111/mmi.13733

Figure Lengend Snippet: E. coli and the indicated deletion mutants from Keio collection (BW25113) or from our laboratory (MG1655) were treated with 100 µg/ml BSA or PGRP for 30 min. The results are means of 3 experiments + SEM; * P<0.05, ** P<0.001, PGRP vs BSA; ^ P<0.05, mutant vs parental strain.

Article Snippet: Bacteria, growth, and media The entire Keio collection of 3884 E. coli K-12 in-frame single-gene knockout mutants was obtained from the National BioResource Project, National Institute of Genetics, Japan ( Baba et al ., 2006 ).

Techniques: Mutagenesis